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Rev. argent. microbiol ; 37(3): 156-160, jul.-sep. 2005. ilus, tab
Article in Spanish | LILACS | ID: lil-634498

ABSTRACT

The aim of this study was to characterize methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered from different infectious sites of hospitalized patients at two university hospitals. Fourteen isolates were analyzed by repetitive sequence based PCR (Rep-PCR), randomly amplified polymorphic DNA assay (RAPD-PCR), and pulsed-field gel electrophoresis (PFGE). We found that a prevalent clone of MRSA, susceptible to rifampin, minocycline, and trimethoprim/sulfamethoxazole (RIF S, MIN S, TMS S) was present in both hospitals in replacement of the multiresistant MRSA South American clone, previously described in these hospitals. The staphylococcal chromosomal cassette (SCCmec) type I element was detected in this new clone.


El objetivo de este trabajo fue la caracterización de aislamientos de Staphylococcus aureus meticilina-resistentes (SAMR), provenientes de diferentes procesos infecciosos de pacientes internados en dos hospitales universitarios. Catorce aislamientos fueron analizados mediante la PCR de secuencias repetitivas (Rep-PCR), la amplificación al azar de ADN polimórfico (RAPD-PCR) y la electroforesis de campo pulsado (PFGE). Encontramos que un clon prevalente de SAMR, sensible a rifampicina, minociclina y trimetoprima-sulfametoxazol (RIF S, MIN S, TMS S) estaba presente en ambos hospitales, reemplazando al clon SAMR y multi-resistente previamente descrito en estos mismos hospitales. En este nuevo clon se detectó el cassette cromosómico estafilocócico SCCmec tipo I.


Subject(s)
Humans , Cross Infection/microbiology , Drug Resistance, Multiple, Bacterial , Hospitals, University/statistics & numerical data , Methicillin Resistance , Staphylococcal Infections/microbiology , Staphylococcus aureus/isolation & purification , Academies and Institutes/statistics & numerical data , Argentina/epidemiology , Bacterial Typing Techniques , Bacterial Proteins/genetics , Cross Infection/epidemiology , Drug Resistance, Multiple, Bacterial/genetics , Electrophoresis, Gel, Pulsed-Field , Methicillin Resistance/genetics , Methicillin/pharmacology , Minocycline/pharmacology , Polymerase Chain Reaction , Random Amplified Polymorphic DNA Technique , Rifampin/pharmacology , South America/epidemiology , Staphylococcal Infections/epidemiology , Staphylococcus aureus/classification , Staphylococcus aureus/drug effects , Staphylococcus aureus/genetics , Trimethoprim, Sulfamethoxazole Drug Combination/pharmacology , Urban Health
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